EKSPLORASI GEN GROWTH HORMONE EXON 3 PADA KAMBING PERANAKAN ETAWAH (PE), SAANEN DAN PESA MELALUI TEKNIK PCR-SSCP

Abstract

This research was conducted to identify genetic polymorphism growth hormone gene of the exon 3 in three goat breeds. Polymorphisms at exon 3 growth hormone gene was identified by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) method. The DNA of 234 goat used were from Etawah Grade (98 samples), Saanen (92 samples) and their crossbred (44 samples) in Cariu, Ciapus, Sukajaya, Cijeruk, Balitnak and Sukabumi. The PCR-SSCP method was performed at 250 V for 8 hours using 12% of acrylamide concentration. The result showed that the annealing temperature is 60C. The PCR product was 157 bp (base pair). The result of SSCP method found four conformational patterns resulting 4 gene types of AA, AB, BC, dan AC. The genotype frequency in exon 3 were AA (0.205), AB (0.856), AC (0.163) and BC (0.045). Further, three alleles were found. The highest frequencies were allele A (0.602) and B (0.443) at Saanen, Etawah Grade and their crossbred goat. The highest genotype frequency was AB at three goat breeds. The highest heterozygosity was found in Etawah Grade, Saanen and their crossbred goat (0.938).