Penghambatan Enzim Siklooksigenase-2 oleh campuran nanopartikel ekstrak Suruhan (Peperomia pellucida) dan Jahe Merah (Zingiber officinale)

Abstract

Both suruhan (Peperomia pellucida) and red ginger (Zingiber officinale) were known to inhibit cyclooxygenase-2 but, the mixture of these two has a low inhibition of cyclooxygenase-2. The aim of this research is to test the potency of suruhan, red ginger, and its mixture in nanoparticle as a cyclooxygenase-2 inhibitor. Groups were divided into suruhan nanoparticle, red ginger nanoparticle, and suruhan-red ginger nanoparticle 1:1, 2:1, and 1:2. Nanoparticle was made using homogenation and ultrasonication. The inhibitory effect was analyzed using Enzyme-linked Immunosorbent Assay (ELISA) principle. The average size of suruhan nanopaticle was 90.61 nm and the Polidispersity Index was 0.22. Average size of red ginger nanoparticle was 173.46 nm and IP was 0.17. Maximum inhibition of cyclooxygenase-2 by nanoparticle of red ginger was 73.82% at 75 μg/mL, and 22.99 % by its mixture at 175.75 μg/mL. Nanoparticle of suruhan did not have an inhibitory effect, however its mixture with nanoparticle of red ginger (1:2) can inhibit cylooxygenase-2.