Synthesis Of Silver Nanoparticles By Using Extracellular Metabolites Of Lactobacillus Delbrueckii subsp. bulgaricus

Abstract

The silver nanopartic/es were used for many applications such as antifungal, anti bacterial, nanosensors, and food wrappers. Synthesis of silver nanopartic/es using microbes as well as metabolites have been developed with consideration of the hazardous waste free environment. The aim of this research was to synthesize silver nanopartic/es by usi~g extracellular metabolites of L. delbrueckii subsp. bulgaricus. The synthesis silver nanopartic/es was carried out by reacting the extracellular metabolites of L. delbrueckii subsp. bulgaricus with silver nitrate (AgNOJ) solution. Reduction of Ag+ ions occurred by interaction of the extracellular enzyme that produced by L. delbrueckii subsp. bulgaricus. The silver nanoparticles was detected by UV-Vis spectrophotometer based on speci.fi.c absorption spectrum at the 400 nm wavelength. The result of Fourier Transform Infrared (FTJR) analysis showed that organic component played an important role in the silver nanoparticles formation. The enzyme was estimated as the organic component in the extracellular metabolites of L. delbrueckii subsp. bulgaricus and had a role in the formation of silver nanoparticles. Furthermore, particles size analysis conformed that average size of formed silver nanoparticles was 2. 7 nm and considered as silver nanoparticles.