Effects Of Extraction Methods On Curcuminoids Contents And Antioxidant Activity Of Curcuma Longa Linn.
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Date
2012Author
Purwakusumah, Edy Djauhari
Kautsari, Sadwika Najmi
Nurcholis, Waras
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Curcuma longa Linn. is widely used in Indonesia ,as an important traditional medicine. Curcuminoids are the mosl important compound of C. longa which is responsible for its antioxidant activity. A comparative study of maceration. microwave, and sonication methods using 70% ethanol as a solvent has been assessed for the antioxidant activity anc curcuminoids content of the fresh and dried rhizome of C. longa . DPPH (l,1-diphenyl-2-picrylhydrazyl) assay was usec for measuring antioxidant activity and spectrophotometry for curcuminoids contents. The 70% ethanol extracts frorr sonication method possessed the highest antioxidant activity and curcuminoids content. The ICso values of antioxidani activity from 70% ethanol extract of fresh and dried rhizome of C. longa were 69.24 to 215.32 and 35.44 to 61.~3 μ~ mL·1 , respectively. The curcuminoids content from 70% ethanol extract of fresh and dried rhizome of C. longa wen 1.89 to 2.45 and 7.17 to 17.91 %. A positive correlation between antioxidant activity and the curcuminoids conten was found in the three of extraction methods.
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