Blood Metabolite statues and immune respone of indigenous sheep related to saponin intake

Abstract

Abstract Saponin is a secondary compound which found some tropical browse plants. It is widely know that saponin may be triggered immune response in animals. The aim of the current sfudy was to evaluate the effect of saponin contents in the tropical browse plants on blood rnetabolites and immune responses in indigenous sheep. Sixteen male growing sheep (average BW of 20 kg) were fed four experimental diets in Compietely Randomized Design. The experimental treatments were cornbined with seventy percentage of native grass and thirfy percentage of each the tropical browse plants (Gliri.cidia sepiunt (GS), Moringa oleifera (MO) and Aftocsrpns heterophyllus (AII)), while the diets contained only native grass was served as control. Dry matter, protein and saponin intake were observed. Blood metabolites including total protein, albumin, globulin and immunoglobulin-G were deterrnined using ELISA. Phagocytosis and ciearance test were done by challenged Staphylococus aureus using total plate count method and evaluated descriptjvely. Dry matter and protein intake were significanfly increased (P<0.05) in sheep fed with GS and AH. Feeding AII was showed the highest the intake of saponin. The experimental treatrnent did not affect the albumin and globulin concentrations in the serum, whereas the levels of total protein in the serum were significantly higher in animals fed MO and AH. The highest Immunoglobulin-G level was observed in animal fed MO which was saponin intake at the values i5 g h-rdt. Phagocytosis capaci6, (360150 cell) and clearance test (2.8 cfu/ml) ehallenge by Stapilococus duleus bacteria also supported that the saponin intake in the ration containing Moringa oledera could improve immune response for the indigenous sheep. The results suggest that supplementation of Moringa olei/bra in the diet with A.75 g kg-1814'-r saponin intake can be used to maintain blood metabolites and healthy status of indigenous sheep.