Vaksinasi Induk Ikan Nila Oreochromis niloticus dengan Sel Utuh dan Ketahanan Benih yang Dihasilkannya Terhadap Infeksi Streptococcus agalactiae

Abstract

The aimed of this study was to evaluate the effectiveness of vaccination on red tilapia broodstock which causes antibodies can be transferred to the offspring. The average weight of broodstocks used in this study were 250 ± 0.03 g were kept in a fiber tube sized 2 m x 1 m x 0.5 m. The study using complete randomized design that consisted of 2 treatments and 3 replications. Female broodstocks were vaccinated using intraperitoneal injections at a dose of 0.4 ml/kg and control fish were injected with Phosphate Buffer Saline (PBS). Observed parameters in this study consisted of mortality, relative survival rate, antibody titers, the ontogeny of kidney tissue. Antibody titers measured using microtiter agglutination method on the broodstock, eggs, body fluids of larva at 5 days, 10 days, and 15 days old. Larval challenge were given at age of 5, 10, and 15 days. The results showed that vaccination of red tilapia broodstock has given significantly different antibody level (P<0.05) in the offspring compared to control treatment with average relative survival rate up to 84, 92 %.