Penentuan Dosis Perendaman Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang pada Pascalarva Udang Galah

Abstract

This research was conducted to determine the immersion dose of recombinant giant grouper growth hormone (rElGH) that generates high growth performance in giant freshwater prawn. The study was consisted of 5 treatments with three replications. Those were immersion dose of 0.15 mg/L, 1.5 mg/L and 15 mg/L. As control post larval-35 (PL-35) was without treatment (control-1) and PL-35 was immersed in water containing 100 mg/L bovine serum albumin (BSA) without rElGH (control-2). In amount of 30 PL-35 for each treatment were bath immersed for 1 hour in a plastic packing containing 200 mL of water and 100 mg/L BSA. PL were further maintained for 12 weeks in the 30-L glass aquarium and fed flake form of commercial diet 3 times daily, at satiation. The results showed that the highest of biomass (58.77±1.98 gram), specific growth (6.85±0.01 % per day), average body weight (1.09±0.01 gram) and average total body length (5.09±1.01 cm) were obtained in 15 mg/L dose of treatment (p<0.05). Biomass of the prawn in 15 mg/L immersion dose was approximately 79.9% higher than control-1 (32.67±1.45 gram). Survival of prawn in all treatment and controls were similar (p>0.05). The prawn immersed with rElGH at a dose of 15 mg/L had 9.55% lower protein content, while lipid content was 6.33% higher than control. Thus, the immersion of PL in 15 mg/L rElGH could increase the growth of giant freshwater prawn, and can be useful to increase the aquaculture production.