Aktivitas Inhibisi Enzim Pengubah Angiotensin pada Hidrolisat Tempe Koro Pedang (Canavalia ensiformis L.) secara In Vitro

Abstract

Tempe is one of the processed food that has ACE (Angiotensin Converting Enzyme) inhibitory activity. This study aims to measure the activity of ACE inhibition in jack bean in vitro. Proximate analysis used AOAC method (2005) and hydrolyzed by gastrointestinal simulation (GIS) digestions, and then tested the degree of hydrolysis by Benjakul & Morrissesey modification method. Measurement of ACE inhibitors, based on the rate of formation of hippuric acid from substrate hippuryl-L-histidyl-L-leusine (HHL). Proximate analysis showed fat value of jack bean is lower than soybean and carbohydrate value is higher than soybean. Degree of hydrolysis (DH) of tempe from jack bean showed good results which amounted to 10.62% for crude tempe and 16.24% for steamed tempe. Of the IC 50 values obtained, crude tempe 50% more effective at inhibiting the activity of ACE by 0.26 mg/g sample. However, for the steamed tempeh also obtain poor results, because of the results obtained IC50 1:11 mg/g sample.