Kinetika Fermentasi dan Kecernaan in vitro Ransum Sapi Potong yang Disuplementasi Probiotik Padat atau Cair

Abstract

The aim of this experiment was to study the effect of solid or liquid probiotic supplementation on in vitro fermentability kinetic and digestibility of beef cattle ration. The fermentability experiment used factorial randomized block design 3×5 with four replicates. Factor A was beef cattle ration without and with supplementation with solid or liquid probiotics: A1 = control ration (rice straw and concentrate, ratio 60:40%), A2 = A1 + 0.25% (w/w) solid probiotic, and A3 = A1 + 0.10% (v/w) liquid probiotic. Factor B was incubation times: 0, 0.5, 1, 1.5, and 2 hours. The digestibility experiment used randomized block design with three probiotic treatments (A1, A2, A3) and four replications. Variables measured were NH3, total VFA, bacterial population, protozoal population, microbial protein synthesis, and digestibility. The result showed that NH3 and total VFA concentrations and digestibility were influenced by probiotic supplementation, whereas bacterial population were affected by probiotic supplementation and incubation time; however, these treatments did not produce significant effects on protozoal populaton and microbial protein synthesis. The result of contrast orthogonal test showed that control ration added with 0.10% (v/w) liquid probiotic is the optimal treatment