Synthesis, Characterization, Stability of Ketoprofen Nanoparticles Basen on Surfactant Type and In Vivo Evaluation of Antiinflammatory.
Sintesis, Karakterisasi, Stabilitas Nanopartikel Ketoprofen Berdasarkan Jenis Surfaktan dan Uji Aktivitas Antiinflamasi Secara In Vivo.
Abstract
In this research, ketoprofen is used as a drug model in the preparation of ihitosan nanoparticles as a potential drug delivery system through ionic gelation wocess with tripolyphosphate (TPP). Suspension stability and characterization of 61itosan nanoparticles-coated ketoprofen prepared by ultrasonication, r+ &ntrifugation, and spray drying were analyzed. §: From the results of Particle Size Analysis, it was shown that the average ~rticle size and polidispersity index of chitosan nanoparticles prepared with oleic i id were 253.7 nm and 0.375 with drug entrapment efficiency of 73.30%, while ~o se prepared with poloxamer 188 were 242.94 nm and 0.302 with drug entrapment efficiency of 87.89%. Scanning Electron Microscopy (SEM) analysis ~owed that the nanoparticles shape, both prepared with oleic acid and poloxamer "lI£8, was intact spherical shape. Fourier transform infrared spectroscopy (FTIR), & wever, indicated several differences between the spectra of chitosan and ~toprofen-loaded chitosan nanoparticles, for example, the peak at wavenumber ~41 cm-1 of ketoprofen-loaded chitosan nanoparticles showed P-OH group of ~P and a new peak at the wavenumber 1409 cm-1 indicated the presence of 'iectrostatic interaction between the carboxyl group of ketoprofen and the amino .group of chitosan. Chitosan nanoparticles suspension prepared with poloxamer 188 showed smaller increase in turbidity and viscosity than that prepared with oleic acid during 34 days of storage both at room temperature and 4°C, which indicates that chitosan nanoparticles prepared with poloxamer 188 is more stable. This stability characteristic is suggested from agglomeration occurred in both chitosan nanoparticles suspensions. Anti-inflammatory activity test of chitosan nanoparticles-coated ketoprofen was perfonned by obeserving the capability to reduce the swelling, or oedema, as a result of injection of 1 % karagenin on the foot pad of rats. The results of the test owed that chitosan nanoparticles-coated ketoprofen had anti-inflammatory ,S¢tivity comparable to the activity of the positive control (uncoated ketoprofen) in ~e same dosage dose, i.e. 43.92% vs 49.90%, after 5 hours of injection .