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      Efikasi vaksin sel utuh Streptococcus agalactiae pada ikan nila Oreochromis niloticus melalui perendaman

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      Date
      2013
      Author
      Febriansyah, Trian Rizky
      Sukenda
      Nuryati, Sri
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      Abstract
      This study aimed to evaluateefficacyof formalin-killed whole cellsof Streptococcusagalactiaecellnon-hemolytic isolate N14G and NK1vaccine givenby bath immersionin preventing streptococcosis in tilapia. The weight of tilapia used that is 10.79±0.99 g, kept as many as 10 fishes in a tank size (60x30x35) cm. The fishes was vaccinated by bath immersion method at concentration 109 CFU/ml. The fishes was challenged by intraperitoneal with 105 CFU/ml at 11 days post-vaccination. Parameters that would observe are survival rate (SR), Relative Persent Survival (RPS), leukocytes total, fagocyte activity, antibody titer, erythrocytes total, haemoglobin value, haematocrit value, dan water quality. The parameters observation held on days 0, 10, 20, and 30. Results showed vaccine treatment that challenged by isolate N14G give the highest survival rate and RPS values than other treatments. Survival rate and RPS both vaccine treatments was 60% and 40%. RPS values were fairly small exhibit vaccinations are less effective in preventing bacterial infections S.agalactiae
       
      Penelitian ini bertujuan untuk mengevaluasi efikasi vaksin sel utuh formalin - killed cell Streptococcus agalactiae tipe non - hemolitik isolat N 14 G dan NK 1 yang diberikan melalui perendaman dalam mencegah penyakit streptococcosis pada ikan nila. Ikan nila yang digunakan memiliki bobot 10,79±0,99 g, dipelihara sebanyak 10 ekor dalam akuarium ukuran (60x30x35) cm. Ikan divaksinasi dengan metode perendaman dengan dosis 10 9 CFU/ml. Uji tantang dilakukan pada hari ke - 11 pascavaksinasi dengan dosis 10 5 CFU/ml. Parameter yang diamati meliputi sintasan (SR), tingkat kelangsungan hidup relarif/ Relative Persent Survival (RPS), total leukosit, aktifitas fagositik, titer antibodi, total eritrosit, kadar hemoglobin, kadar hematokrit, dan kualitas air. Pengamatan parameter dilakukan pada hari ke - 0, ke - 10, ke - 20, dan ke - 30. Hasil penelitian menunjukkan perlakuan kedua vaksin yang diinfeksi bakteri isolat N 14 G memberikan nilai sintasan dan nilai RPS tertinggi dibanding perlakuan lainnya. Nilai sintasan dan RPS kedua perlakuan tersebut adalah 60% dan 40%. Nilai RPS yang cukup kecil menunjukkan vaksin yang diberikan masih kurang efektif untuk mencegah infeksi bakteri S. agalactiae.
       
      URI
      http://repository.ipb.ac.id/handle/123456789/64017
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      • UT - Aquaculture [2191]

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