The Effect of Secang Wood (Caesalpinia Sappan Linn) Extracts on the Mouse Splenocytes and K-562 Cells Proliferation

Abstract

Secang wood is used as a raw material in many traditional medicines and healthy drinks. The specific component brazilin, an isoflavone, is known to have antioxidant and antibacterial activities. Phenolic compound which has antioxidative activity is expected that it can protect cell from damage and death. On the other hand, some of phenolic compunds had been proven that it had antiproliferative activity and toxic effect to cancerous cell. The aim of this research was to know the effects of secang wood extracts on the cell proliferation in vitro, especially mouse splenocyte and K-562 cell line. This study showed that all of supplemented levels (0.1 , 1,5, 10 and 20 x C; which C was estimated equivalent to daily consumption dosage) of secang wood extracts (methanol, ethanol and water extract) resulted in higher splenocytes proliferative index than that of the control (the cells without any secang wood extracts added). The methanol 01 extract resulted in the highest proliferative index, followed by ethanol extract and then water extract. At low supplemented level (0.1 x C), the methanol, ethanol and water extracts showed to depress K-562 cells proliferation, but at the higher levels they were not toxic to the cells. Methanol and ethanol extracts, however, demonstrated very highly proliferative. This result suggested that there was a growth factor compound in secang wood extract which was high soluble in methanol and ethanol