Analisis Keragaman Genetik Bakteri dalam Teknologi Budidaya Bioflok dengan Teknik ARDRA gen 16S-rRNA

Abstract

A new approach to reducing farmed waste is aquaculture with biofloc technology (BFT-Bio-flocs technology). Bacteria is the main constituent in aquaculture systems with biofloc. This study aimed to analyze the genetic diversity of bacteria biofloc using 16S-rRNA PCR with ARDRA technique. A total of 38 dominant bacterial isolates was obtained and of these isolates, 16S-rRNA gene was then isolated and amplified using PCR. The 16S-rRNA gene of the isolates was then cut using HaeIII (5’-GG•CC) and HhaI (5’-GCG•C) restriction enzymes resulting an ARDRA pattern which was further used as the binary data for the construction of phylogenetics tree that can be used to estimate the group of bacteria. The result with HaeIII cutting restriction enzyme from bacteria bioflocs obtained 11 ARDRA patterns, while with the restriction enzyme HhaI obtained 8 ARDRA patterns. Phylogenetics of bacterial populations on tree water biofloc cultivation system consisting of at least 13 different bacterial species. Result of sequencing from 2 gene sample 16S-rRNA identified Microbacterium foliurum, and Pseudomonas putida.