Isolasi Oligosakarida Madu Lokal dan Analisis Aktivitas Prebiotiknya

Abstract

Oligosaccharides are the most prebiotic studied, and are natural component of honey. Sumbawa and Kalimantan forest are local honey producers in Indonesia but its oligosaccharide content and prebiotic had never been investigated yet. Oligosaccharide isolated with activated charcoal adsorption and ethanol treatment. Effectiveness of this method is evaluated by Thin Layer Chromatography and colorimetry. The isolates then subject to prebiotic examination. Honey from Sumbawa forest has higher oligosaccharides content than honey from Kalimantan. Qualitative and quantitative assays showed oligosaccharide isolation method were effective to concentrate the oligosaccharide with high degree of polymerization but ineffective for mono- and disaccharides removal. Percentages of hydrolysis of oligosaccharide from Sumbawa and Kalimantan by mimic gastric juice were 1.78% and 0.59 %. Whereas, the hydrolysis was higher with amylase, 11.87% for Sumbawa and 21.03% for Kalimantan honey oligosaccharides. Prebiotic activity was 0.32, and 0.09 for honey oligosaccharide from Sumbawa and Kalimantan respectively. Honey oligosaccharide from Sumbawa presented higher prebiotic activity than inulin as prebiotic reference (0.11). GC-MS chromatogram showed L. acidophilus cultured in media added with Sumbawa honey oligosaccharide produced lactic acid as the highest metabolite (48.01%).