Aplikasi Marka Aromatik Bradbury dan RM 223 untuk Identifikasi Hasil Persilangan Ciherang-Mentik Wangi dan Ciherang-Pandan Wangi.

Abstract

Rice as a basic commodity can be distinguished between aromatic and nonaromatic. Its different based on gene deletion at chromosom 8. Conventional methods like assessment was frequently uses to detect aromatic characteristic of rice. Gene based molecular was a new method to identify aromatic rice accurately and shorthen analyzing time. The purpose of this research were to select and to get rice F1 Ciherang-Mentik Wangi use Bradbury primer and BC1F1 Ciherang- Pandan Wangi use RM223 primer. DNA as rice genetic material were isolated from leaves using Cetil Trimetil Amonium Bromida (CTAB) method which broke the cell wall and release the components, thus precipitated the DNA. DNA amplification conducted by PCR machine using specific aromatic primer and the result verified by 1.5 % and 3% gel agarose electrophoresis. The result of amplification by using Bradbury primer of F1 Ciherang-Mentik Wangi obtained size of band about 580 bp and 355 bp for nonaromatic, also 580 bp and 257 bp for aromatic. Furthermore, RM223 primer can be differentiate both of patterns of aromatic and nonaromatic rice by the band size about 120 bp to 160 bp.