Pembentukan dan Seleksi F1 Padi Ciherang-Pandan Wangi dan Fatmawati-Mentik Wangi Menggunakan Marka Aromatik

Abstract

Development of aromatic rice varieties have been carried out by using genetic engineering approach. Nevertheless, commercialization of those genetic modified rice will require the strict genetic modified organism regulation. The objectives of this experiment were to study of introduction of aromatic trait from aromatic into nonaromatic rice through crossing and to screen the F1 plants by using molecular markers linked to aromatic trait. Pandan Wangi and Mentik Wangi will be used as aromatic donor parents meanwhile Ciherang and Fatmawati are as recurrent parents. Pandan Wangi will be crossed with Ciherang dan Mentik Wangi will be crossed with Fatmawati to produce aromatic F1 hybrids. PCR-based markers that will be used in this research i.e. Bradbury primer for detecting the aromatic trait in F1 Fatmawati x Mentik Wangi and RM223 primer for F1 Ciherang x Pandan Wangi. Result of the research showed that F1 hybrid plants have been obtained from both of the crossing combination. Screening of the F1 plants showed that the tested F1 plants have been proved carrying the aromatic trait indicated by the formation of DNA band heterozygous with size 580, 355, and 257 bp for F1 plants Fatmawati x Mentik Wangi and 140 and 160 bp for F1 Ciherang x Pandan Wangi.