Ekspresi Gen Aminocyclopropane Carboxylic Synthase pada Klon Tanaman Karet Akibat Pemberian Etefon

Abstract

Stimulant treatment using etephon is one way to increase production of natural rubber. Etephon will affect gene expression of Hb ACS, a gene coding for the enzyme. The research aimed to isolate and compare the gene expression patterns of Hb ACS due to the provision 2.5% etephon on the leaves and bark of rubber tree clones PB 217, GT 1 and PB 260. The samples that already treated with 2.5% etephon were collected at the time 0 until 8 hours, then the RNA were isolated and analyzed of gene expression by RT-PCR. Etephon treatment capable to affects the expression of Hb ACS-F2 and Hb ACS-E4 on PB 217 clone at the maximum observed in the leaves between 2-4 hours and 6-8 hours on the bark. Hb ACS-D10 expression in the leaves was observed at the 2nd and 6th hours, while in the bark was observed on the 8th hour. Hb ACS-F2 and Hb ACS-E4 expression on GT 1 clone were observed at the maximum on the leaves after 4-8 hours, while in the bark the expression of Hb ACS-F2 was observed more uniform until 8 hour and the Hb ACS-E4 fragment was not expressed. The Hb ACS-F2 expression on PB 260 clone maximum observed on leaves at 0, 4th, and 8th hours, while in the bark was observed after 2 hours maximum. The Hb ACS-E4 expression on PB 260 clone was observed more uniformly on the leaves until 8th hour, while in the bark maximum observed at 0 hours after application etephon. The Hb ACS-D10 expression on GT 1 and PB 260 clones were not observed.