Potensi Antioksidan dan Sitotoksisitas Ekstrak Buah Ceremai (Phyllanthus acidus L.)

Abstract

Ceremai is an indigenous plant from India, belongs to Euphorbiaceae family. Ceremai plnat not only be used as an ornamental plant but can also be used as a herbal supplement. Ceremai Plants reported to have efficacy as a hepatoprotective, antibacterial, antifungal, antioxidant potency is not known yet. The purpose of this study to prove the antioxidant activity and cytotoxicity of fruit Ceremai. Ceremai was extracted by maceration using 70% ethanol, 30% ethanol, and water as solvents. Results were determined by antioxidant activity using of 2,2-diphenyl-1-pikrilhidrazil (DPPH) and its cytotoxicity (biological potency) was determined by Brine Shrimp Lethality Test (BSLT) method. IC50 values were 26.06 ppm, 72.39 ppm, and 62, 17 ppm for water, 30% ethanol and 70% ethanol exstract respectively. LC50 values were produced from three extracts water, ethanol 30%, and 70% ethanol, they were 473.26 ppm, 486.78 ppm, 618.55 ppm. Water extract is the best antioxidant activity compared with 30% ethanol extract and 70% ethanol. However, ceremai fresh fruit extracts are not good for human consumption herbal supplements because it is toxic.