Bioaktivitas Antioksidan dan Antiinflamasi Secara In Vitro serta Kandungan Kurkuminoid Temulawak dan Kunyit Asal Sukabumi

Abstract

Curcuma xanthorrhiza and Curcuma domestica are kind of medicinal plants which have many health benefits. Both of them have curcuminoid content which is useful as an antioxidant and anti-inflamatory. In this study, ethanol extracts of C. xanthorrhiza and C. domestica promising lines from Sukabumi, Indonesia were investigated for the presence of curcuminoids, antioxidant and anti-inflamatory activities. HPLC method were used to determined curcuminoids content. The antioxidant (radical scavenging) potential of the samples was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method. While for the anti-inflamatory activity, the in vitro cyclooxygenase 2 (COX2) inhibition method was used. The curcuminoid content of C. xanthorrhiza and C. domestica were 31.27 mg/g and 66.32 mg/g, respectively. IC50 values for DPPH radical scavenging activity were 81.99 μg/mL and 73.31 μg/mL, with C. domestica having lowest value and most potent than C. xanthorrhiza. Percent inhibition values for COX2 inhibitor activity were 74.84 % and 67.96 %, with C. domestica having the highest value. In this study, the ethanol extracts of C. domestica promosing line from Sukabumi of Indonesia exhibited most in curcuminoids content, antioxidants properties and anti-inflamatory activity than C. xanthorrhiza promosing line.