Isolasi Dan Identifikasi Bakteri Petrofilik Dari Konsentrasi Residu Total Petroleum Hidrokarbon (Tph) Di Bawah 1% (W/W) Hasil Proses Bioremediasi (The Isolation And Identification Of Petrofilic Bacteria From Total Petroleum Hydrocarbons (Tph) Residues Under 1% (W/W) Of Bioremediation Process Results)

Abstract

In minister of decree (Keputusan Menteri Lingkungan Hidup No.128/2003) mentioned that the final requirement of the recovery contaminated land by Total Petroleum Hydrocarbon (TPH) through the bioremediation is less than 1%. The TPH residues resulted from bioremediation are difficult to degrade naturally and requires a treatment process in long term, while the treatment process output quantity is increasing. Thus, these effects will bring new problems for environment. The isolation and identification of petrofilic bacteria is the beginning phase of this research and will be continued by determination of biodegradation kinetic parameters and examine the biodegradation process at landfarming reactor on a laboratory scale. The purpose of this research is finding the bacteria isolates that have high affinity towards the given substrates. The bacteria isolation media was using Solution Base Salt (SBS) which enriched with yeast extract and crude oil. The isolation phase produced 6 single colonies of bacteria isolates that potentially degrading crude oil with clear zones characteristics around the colonies. The identification phase produced Pesudomonas putida AK.A (4 isolates) and Pseudomonas diminuta AK.B (2 isolates). These bacteria are gram-negative bacteria, aerobic, rod-shaped, and can degrade the pollutants especially aromatic hydrocarbons. Both of these isolates will be used to determine biodegradation kinetics of both in a single colony or a mixed culture.