Effects of Dietary Supplementation of Natural Feed Additive on Leucocyte Profile and Lymfoid Organ of Broiler

Abstract

Herbal, probiotic and prebiotic as natural feed additive have potential to replace antibiotic in poultry diet. This experiment was conducted to study the effect supplementation of herbal, probiotic, prebiotic and synbiotic in the diet on leucocyte profile and lymfoid organ of broiler. The experiment used 180 day- old Ross broiler chicks (unsexed) which were reared for 5 weeks. This experiment used completely randomized design with 6 dietary treatments and 3 replications. The dietary treatments were : 1. Basal diet as negative control (without feed additive), 2. Basal diet + antibiotic (bambermycin 0.05%) as positive control, 3.Basal diet + probiotic (EM4, 1cc/l), 4. Basal diet + prebotic (fermacto, 0.2%), 5. Basal diet + herbal mixed (Curcuma longa, Curcuma xanthorriza and Zingiber officinale, 1.5%), 6. Basal diet + synbiotic (EM4 , 1cc/l and Fermacto, 0.2%). At the end of feeding trial (5 weeks of age), 6 birds each treatment were sacrificed to measure the lymfoid organ (sleen and bursal fabricius). Blood sample were collected for heterofil and lymfosit measurement. There were no significant difference on spleen, heterofil (H) and lymfosit (L) due to dietary treatments. However, bursal fabricius of birds fed antibiotic, probiotic and synbiotic diets significantly (p<0.05) lower than birds fed negative control diet. Birds fed probiotic diet had lowest mortality as compared to other treatments. In conclusion, probiotic supplementation gives better health performance than other natural feed additive.