Interaksi Kultur Tunas In Vitro Aquilaria Spp. Dengan Hifomiset (Acremonium Spp. Dan Fusarium Spp.) Asal Bangka

Abstract

Agarwood resin was formed as a response of agarwood tree (A qui/aria spp.) to hyphomycetes infection. Eight isolates of hyphomycetes (5 isolates Acremonium spp. and 3 isolates Fusarium spp.) from Sangka's agarwood were interacted with in dual culture of shoot Aqui/aria crassna klon AC8, A malaccensis klon PO, A microcarpa klon CD in media 50% modifiedMurashige- Skoog agar. Hyphomycetes response towards the presence of shoot and shoot response towards hyphomycetes, fragrance index and the presence of terpenoid substance were observed for 4 weeks interaction. The presence of shoot affected the growth of hyphomycetes. All hyphomycetes caused all clones of the shoot death at fourth week of interaction. Acremonium L, Fusarium A, S, and 0 induced fragrance formation. The fragrance level was in moderate level (score 1 out of 3) of those isolates, Acremonium L might induce fragrance formation in all clones of shoot. Sterol was detected both in interacted and control shoots of Amalaccensis and A microcarpa.