Kajian awal produksi aseton-butanol-etanol secara sinambung dari substrat hidrolisat tandan kosong kelapa sawit (elaeis guineensis, JACQ) dalam bioreaktor unggun diam

Abstract

The aceton-butanol-ethanol (ABE) has become one of the prospective alternatives, in order to handle empty fruit bunches of oil palm (EFBO) accumulation, and self supply of the organic solvent tor Indonesian industries consumption Because of its intrinsic compounds, the effective hydrolysis method of EFBO will determine the feasible hydrolyzate for ABE fermentation. In this study, the hydrolysis of EFBO was carried out in two stages Delignification was carried out by using the method of acid hydrolysis on the sugar production from empty fruit bunches of oil palm. Which selled the particles of EFBO (60 mesh) by NaOH 1 N for two hours, followed by autaclaving(12rC, 15 minutes) and enzymatic hydrolysis by cellulose for 72 hour sunder pH 4.8. during hatch fermentation, using 13.89 g/l fermentable sugar of EFBO hydrolyzate and 6.11 g/l glucose, the mmax was found at 0.202 hour while using 60 g/l sucrose the mmax found was 0.079 hour. The continous fermentation of EFBO hydrolyzate (adjusted to 40 g/l fermentable sugar) was carried out using packed bed bioreactor and silica gel matrices in three dilution rates (0.05, 0.10, 0.15 hour). After the steady state (maintained for at least three retention times), the high productivity of ABE (1.57 g/l hour) was shown at a dilution rate of 0.15 hour.