Aktivitas antibakteri kitosan berdasarkan perbedaan derajat deasetilasi dan bobot molekul

Abstract

Chitosan is a derivatives product of chitin that can be found as waste from fishery industries. The application chitosan as antibacterial agent is affected by the degree of deacetylation and molecular weight. Increasing the value of deacetylation degree and decreasing its molecular weight can be done by hydrolisis of chitosan using sodium hydroxide. This effort can be use to increase the antibacterial activity of chitosan. The method to determine deacetylation degree can be done by Fourier Transform Infrared, molecular weight by viscometry, and bacterial activity by well difusion method. Hydrolisis of chitosan showed an increased of degree deacetylation up to 21.18% and molecular weight decrease to 63.33%. Based on antibacterial activity to E. coli and S. aureus, after hydrolisis the activity were higher. Compared to chitosan before hydrolisis, the difference of degree deacetylation and molecular weight influence the antibacterial activity.