Sensitivitas gen sitokrom B (Cyt b) sebagai marka spesifik pada genus Rattus dan Mus untuk menjamin keamanan pangan produk asal daging

Abstract

Appropriate techniques for detection and identification of origin of species for meat source are very important in the meat product processing to ensure the safety and halal foods. Multiplex PCR, in which many primers were used together for amplification of multiple target regions, is hopefully a suitable technique for meat sources identification. The aim of this study was to determine the specificity and sensitivity of the cytochrome b (cyt b) gene in rats as well as to design primers that can be used for detection of rat meat contamination in some processed meat products. The amplification of cyt b genes was done in seven species with different lengths of fragments which indicated specificity for cyt b genes sequences between species. The products showed specific DNA fragments of 157, 227, 274, 331, 398, 439 and 603 bp from goat, chicken, cattle, sheep, pig, horse and rat meats, respectively. Cyt b gene is sensitive as a specific marker with rats DNA that could be detected and be amplified with 100% success from as low as 1% level of contamination. Results showed that rat DNA in meatballs could be detected from 1% level contamination, otherwise rat DNA in meat floss could be detected from above 2.5% level contamination.