Aktivitas antibakteri ekstrak kasar flavonoid daun gambir (Uncaria gambir Roxb)

Abstract

Infection of diseases could be caused by Staphylococcus aureus and Eschericia coli that can be prevented by antibiotics. The use of antibiotic in high concentration causes some strains of S.aureus and E.coli become resistant. This situation will encourage the exploration of new antibacterial sources that are available in large quantities and lower resistance effect. The solution is to utilize the antibacterial potency of the leaves of gambir. The objective of this study is to examine the antibacterial activity of crude extract of flavonoids in gambir leaves. Extraction of flavonoids have been done by maceration storey method and obtained aquous extract, chloroform extract and hexane extract. The potential of those extracts have been examined to inhibit the growth of E. coli dan S. aureus by agar diffusion method. The results showed that aquous extract had antibacterial activity. Based on the phytochemical analysis, the aquous extract contained flavonoid. Bacteriostatic was determined by agar diffusion method, meanwhile the bactericidal was determined by measurement of the optical density of minimum inhibition concentration (MIC) at wavelength of 600 nm. The crude extract of flavonoids in gambir leaves are bacteriostatic against Eschericia coli and Staphylococcus aureus at concentrations of 1%, 3%, 5% and 7%. Inhibition against Eschericia coli and Staphylococcus aureus occurred at concentration of 1% and classified as weak, while the concentration of 3%, 5% and 7% was classified as strong. The crude extract flavonoid have bactericidal activity at concentration 9%.