Potensi nanopropolis lebah madu trigona spp asal pandeglang sebagai antibakteri

Abstract

Propolis is one of the natural produced by honey bee which have many benefits, due to its properties as antibacterial, antivirus, and anticancer. Use the form nanopropolis expected to provide a better antibacterial activity compared to the usual form of propolis. The aim of this study is to determine potency of nanopropolis honey bee from Pandeglang as antibacterial agent. Encapsulated nanopropolis was prepared by high speed homogenization technique and solvent evaporation. The size of nanopropolis was measured with SEM. The antibacterial activity test was performed by agar well diffusion. The result of SEM show that size of nanopropolis is 175-873 nm. Nanopropolis have activity as antibacterial agent for Gram positive dan Gram negative bacteria. The nanopropolis showed higher bacterial activity than extract propolis were 208.86% for B. subtilis, 211.83% for S. aureus, 227.01% for E. coli, and 230.29% for Salmonella sp. While compared to amphicillin 10 mg/ml equel to 43.87%, 49.12%, 42.35%, and 37.58%. Minimum Inhibitory Concentrartion (MIC) of nanopropolis were 0.15%, 0.075%, 0.15%, and 0.313% for B. subtilis, S. aureus, E. coli, and Salmonella sp.