Isolasi dan pemurnian bahan aktif dari mikroalga BTM 11 sebagai inhibitor RNA helikase virus hepatitis C

Abstract

Hepatitis C virus is the cause of hepatitis C disease which has high virulence. Recent therapy using combination of ribavirin and alpha interferon has short efficiency < 80%. Thus, the discovery of new drug is needed. Antiviral drugs can be discovered through molecular target therapy by finding the inhibitor of RNA helicase that play role in viral replication. Inhibitor can be derived from chemical compound produced by microalgae. The aim of this research was to isolate the active compound from microalgae BTM 11 which had inhibitory activity against RNA helicase. Inhibitory activity was measured by releasing of phospate inorganic in colorimetric ATPase assay. Crude extract was fractionated using gel filtration chromatography with methanol in chloroform solvent. The result showed that Fraction 3 has the highest inhibitory activity which (81.205%). Phytochemical test of crude extract indicated positive flavonoids and coumarin. Thin layer chromatography (TLC) analysis with eluent chloroform: methanol gave 2 spots with Rf value of 0.8 and 0.37. Moreover, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 minute and 16.617 minute was 266 nm and 230 nm. According to these of phytochemical, TLC, and HPLC, inhibitor compound from BTM 11 belongs to flavonoids.