Aplikasi PCR berbasis marka sub1 (AEX1 dan RM219) pada seleksi padi BC1F1 Ciherang-Sub1

Abstract

Submergence stress is the third most important limitation to rice production from 42 biotic and abiotic stresses. FR13A has adapted to excessive flooding environment by submergence tolerance is controlled by a single major quantitative trait locus (QTL) on chromosome 9, named Sub1. However, these varieties are susceptible to diseases and insect pests. It is therefore an attractive research proposition to transfer Sub1 gene from traditional cultivars into more productive varieties. In this study, the Ciherang was used as host, and F1 Ciherang-Sub1 was used as donors Sub1. The purpose of this study were to generate BC1F1 Ciherang-Sub1 varieties from backcross between F1 Ciherang-Sub1 and Ciherang by site-directed crossing methods. Selection of BC1F1 was done by submergence test and PCR analysis based Sub1 marker. BC1F1 Ciherang-Sub1 was succed in producing and contain Sub1 gene are obtained 16 BC1F1 Ciherang/Swarna-Sub1 and 22 BC1F1 Ciherang/IR64-Sub1 plants. Results of PCR amplification based AEX1 showed DNA band for Sub1A gene at 231 bp. The rices were containing DNA band belong to submergence tolerance rices. RM219 marker could distinguished between Ciherang, Sub1 donors, and BC1F1 Ciherang-Sub1 with DNA fragment at 204 bp and 194 bp. However, the different is too small, it is ranges of 5-10 bp until difficult to determine clearly heterozygous of BC1F1 Ciherang-Sub1.