Persistensi vaksin DNA penyandi glikoprotein 25 yang diberikan melalui pakan buatan pada ikan mas (Cyprinus carpio)

Abstract

Koi Herpes Virus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak in almost all centre of common carp culture in Indonesia and caused mass mortality. DNA vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. In this research, persistence test of DNA vaccine (pMBa-GP25) were carried out by oral feeding on common carp. DNA vaccine persistence test were done using PCR method with the specific primer for GP25 gene. Escherichia coli DH5α bacteria containing pMBa-GP25 DNA vaccine were cultured in four treatment mediums namely 2xYT, LB polypeptone, LB triptone and TSB. Bacteria densities were counted with spectrophotometer using 580 nm wave lengths. The results showed that an efficient culture media for E. coli was LB tripton. Furthermore, GP25 DNA was detected in kidney and spleen of vaccinated fish. Level of DNA vaccine that could be detected was 7,56 ng (amount of DNA copy 1,598 x 1010 copies).