Ekstraksi dan analisis fitosterol lembaga gandum (Triticum sp.)

Abstract

Phyfosterol may reduce the absorption of cholesterol, and used for preventing atherosclerosis. It is limited in soyaean, but poientially abundant in wheat germ. Research on the utilizalion of wheat germ sterol had not been reporled so far. Many aspect: of germ sterol extraction from wheat germ and its characteristics were still unknown. In this research, the best extraction method, kinds and content of phytosterol from wheat germ were investigated.

phylosterol may reduce the absorption of cholesterol, and used for preventing atherosclerosis. It is limited in soybean, but potentially abundant in wheat germ. Research on the utilization of wheat germ sterol had not been reported so far. Many aspects of germ sterol extraction from wheat germ and its characteristics were still unknown. in the research, the best extraction methode, kinds and content of phytosterol from wheat germ were investigated. This research consisted of two steps: (1) extraction of phytosterol directly from whole germ and ground germ using hexane, and indirect extraction through germ oil using hexane and mixed solvent of hexane and ethanol, and direct extraction from ground germ using ethanol; (3) analysis of the type and content of phytosterol in the crude extract through the following steps; preparation of crude extract, fractination, and analysis.