Konstruksi Padi Nonaromatik yang Beraroma Wangi Menggunakan PCR Berbatuan Marka Gen BADH2

Abstract

The high commercial value and maket demmand of fragrant rice have triggered a number of researchs related to fragrant in rice. Various fragrant specific markers have been reported (Bradbury et al. 2005b, Lang and Buu 2008, Shi et al. 2008, Sakthivel et al. 2009). However, except for fragrant property, other agronomic traits (productivity; geographical location and period of cultivation; stress, insects, and disseases tolerance; simplcity of cultivation and maintenance, etc.) of non-fragrant rice are disadvantages compare to those of fragrant rice. These have become barriers for farmer to grow fragrant rice. Therefore, Ciherang rice possesing native agronomic traits and additional fragrant property, was developed in this research. To avoid transgenic plany product, construction was carried out through site-directed crosing and using badh2 specific marker-assisted PCR for progeny selection (Bradbury et al. 2005b). In the first year (2009), DNA of non- fragrant Ciherang and fragrant (Mentik Wangi, Gilirang, Pandan Wangi) rice were isolated from leaves and PCR amplified following the method as described by Shure et al. (1983) and Bradbury et al. (2005b), respectively. Fragrant rice with distinct PCR profile than that of non-fragrant Ciherang rice was then crossed to Ciherang. F1 seeds were planted, their DNA were isolated, and PCR analyzed as previous. The selected heterozygous F1 were then backcrossed to Ciherang, and the obtained BC1F1 were planted and analyzed as described for Fl. PCR results showed that only Mentik Wangi pofile was disticnt compare to that of Ciherang, and therefore was selected as fragrant donor. In Fl progeny (Ciherang-Mentik Wangi) selection, heterozygot Fl with band amplification of Ciherang and Mentik Wangi, as described by Bradbury et al. (2005b), were obtained. The same results were obtained in BC1F1 (Ciherang-Mentik Wangi) progeny selection. In the 2nd (2010) and the 3rd (2011) year of research, BC1F1 will be further backcrossed 4 x and selfed, to obtain homozygous BC5F2 (Ciherang possesing Mentik Wangi fragrant).