Pah-degraders marine bacteria isolated from chronically contaminated sendiment by petrolium hydrocarbons

Abstract

The main purpose of study was conducted to isolate PAHs-degraders strain from Donan river mangroves ecosystem and to investigate the ability of isolated pure culture to degrade P AHs. The potelltial use of these marine bacteria as the environment clean-up agents was conducted by sublimizing with the 6 different Polyaromatic hydrocarbons (PAHs) compounds as a contaminant model such as phenothiazine, fluorene, fluoranthene, dibenzothiophene, phenathrene, and pyrene. The 16S rDNA amplification using primer 9F and 151 OR has been applied and purification was made on the agarose (l %). Sequenced results were obtained by comparing to NCBI Blast. Three rods shape Gram-positive endospore-forming bacteria were isolated from a mangrove site which is chronically contaminated from petroleum hydrocarbons. On the basis ofphenotypic and phylogenetic data, three strains should be placed in the genus Bacillus as three distinct species, for which the names Bacillus aquimaris, Baciiius megaterium, and Bacillus pumilis are proposed. The other three strains were flexibactereae bacterium, Halobacilus trueperi, and Rhodobacteraceae bacterium.