The Effect of Garlic (Allium Sativum) Extract on The Growth of Bacteria Isolated From Uterus Dairy Cattle

Abstract

Many dairy farms suffered from tremendous economic losses due to repeat breeding. Laboratory investigation demonstrated that several bacteria. i. e., Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas sp., and Klebsiella sp. were responsible for this syndrome. This research was to promote the uses of traditional medicinal herb as an alternative way to cure repeat breeding. Garlic was as well known as a herb containing antibacterial effects. The objective of this study was to asses the effects of garlic extracts on the growth of bacteria isolate which were collected from repeat breeding infected of local milking cows. A factorial design 3 x 4 was employed for this investigation. Four isolates of bacteria from repeat breeding, i.e., Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas sp., and Klebsiella sp. were used in in-vitro test. Fifteen micro liters each of three concentrations of garlic extract, i.e., 75, 50, and 25% were dropped in sterile paper disks.The disks were than laid on the MEU blood media previously inoculated with each of the four isolates and were incubated overnight at 37 degree C. The finding results showed that that the highest concentration of garlic extract inhibited bacterial growth In further results showed that the garlic extract significantly inhibited the growth of Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas sp., and Klebsiella sp. [p<.0001]. As conclusion, the Garlic (Allium sativum) extracts had bactericide effects, especially on Staphylococcus aureus, and Streptococcus pyogenes; the is positive correlation between the high concentration of garlic extract with inhibition zones; Staphylococcus aureus shown the most affected by the Garlic extracts at all concentrations and Garlic extract was able to use effectively for controlling the bacteria growth.