Identification of Growth Hormone (Gh) Gene MspI and AluI Loci Polymorphism in Beef Cattles

Abstract

The research was conducted in order to identify the polymorphism of MspI and AluI locus of the growth hormone (GH) gene in Bali, Limousine and Simmental cattle. Total blood samples of 87 cattle were taken from population of Bali cattle were collected from Balai Pembibitan Ternak Unggul Sapi Bali in Bali island, whereas Limousine and Simmental cattle blood samples were collected from Balai Inseminasi Buatan (BIB) Singosari-Malang, West Java. PCR-RFLP and sequencing methods used to detect the polymorphism and mutation at MspI and AluI loci of GH gene. The results showed that at MspI locus, the Bali, Limousine and Simmental cattle had one genotype (-/-), three genotypes(+/+, +/- , -/-) and two genotypes (+/+, +/-), respectively whereas for AluI locus, the Bali, Limousine and Simmental cattles had one genotype (LL), two genotypes (LL, LV) and three genotypes (LL, LV, VV), respectively. The allele frequencies of + and – alleles in Bali, Limousine and Simmental cattle were 0.000 and 1.000; 0.636 and 0.364; 0.889 and 0.111 respectively, whereas the frequencies of L and V alleles in Bali, Limousine and Simmental cattle were 0.000 and 0.000; 0.818 and 0.182; 0.694 and 0.306 respectively. Based on polymorphic informative content (PIC) value, it can be concluded that MspI and AluI loci in Bali cattle are monomorphic, while in Limousine and Simmental cattle is polymorphic. Based on the sequencing results, the MspI (+/+ and -/- genotypes) and AluI (LL and VV genotypes) loci showed a occurrences of nucleotide base mutation from cytosine (C) to thymine (T) and cytosine (C) to guanine (G), respectively.