Pengaruh Pelarut Ekstraksi Rimpang Temu Ireng Metode Microwave Assisted Extraction Terhadap Kandungan Polifenol dan Kapasitas Antioksidan

Abstract

Temu ireng (Curcuma aeruginosa) merupakan salah satu tanaman yang mengandung senyawa fenolik berupa flavonoid yang berpotensi sebagai antioksidan alami. Pemilihan pelarut ekstraksi yang tepat merupakan hal yang penting untuk mendapatkan metabolit sekunder berkualitas. Penelitian ini bertujuan menentukan pelarut terbaik untuk mengekstrak rimpang temu ireng berdasarkan kadar fenolik total, kadar flavonoid total, dan kapsitas antioksidan dengan metode 2,2-diphenyl-1-pycrilhidrazil (DPPH) dan Ferric Reducing Antioxidant Power (FRAP). Kadar fenolik total diukur menggunakan metode Folin-Ciocalteu dan kadar flavonoid total diukur menggunakan metode Alumunium Klorida. Sampel diekstraksi dengan metode microwave assisted extraction (MAE) dengan pelarut akuades, etanol 50%, metanol 50%, dan aseton 50%. Hasil penelitian menunjukkan pelarut aseton 50% memiliki kadar fenolik total tertinggi (3,10 mg GAE/g BK), sedangkan pelarut metanol 50% menunjukkan kadar flavonoid total tertinggi (4,10 mg QE/g BK), serta kapasitas antioksidan DPPH (1,28 µmol TE/g BK), dan FRAP (12,21 µmol TE/g BK). Pelarut terbaik dalam mengekstrak senyawa aktif pada rimpang temu ireng adalah metanol 50% karena menghasilkan kadar flavonoid total serta kapasitas antioksidan metode DPPH dan FRAP tertinggi.

Curcuma aeruginosa is one of the plants that contain phenolic compounds in the form of flavonoids that have the potential to be natural antioxidants. Choosing the right extraction solvent is essential to obtaining quality secondary metabolites. This study aimed to determine the best solvent to extract black rhizomes based on total phenolic content, total flavonoid content, and antioxidant capacity by DPPH and FRAP methods. The total phenolic content was measured using the Folin-Ciocalteu method and the total flavonoid content was measured using the Aluminum Chloride method. The samples were extracted by microwave assisted extraction (MAE) method with aqueous solvents, 50% ethanol, 50% methanol, and 50% acetone. The results showed that 50% acetone solvent had the highest total phenolic content (3,10 mg GAE/g BK), while 50% methanol solvent showed the highest total flavonoid content (4,10 mg QE/g BK), as well as the antioxidant capacity of DPPH (1,28 µmol TE/g BK), and FRAP (12,21 µmol TE/g BK). The best solvent for extracting the active compounds in the rhizomes of black temu is 50% methanol because it produces the total flavonoid levels and the highest antioxidant capacity of the DPPH and FRAP methods.