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      Total Fenolik, Total Flavonoid, dan Aktivitas Antioksidan Ekstrak Teh Hijau, Teh Hitam, dan Teh Putih Kombucha

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      Date
      2024
      Author
      Suantari, Ni Gusti Ayu Made Dewi
      Purwanto, Ukhradiya Magharaniq Safira
      Safithri, Mega
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      Abstract
      Kombucha tea is a fermented drink made from tea containing sugar by kombucha culture or SCOBY (Symbiosis Culture of Bacteria and Yeast). Total phenolic, total flavonoid and antioxidant activity tests were carried out to analyze and compare the antioxidant ability of kombucha made from green, black, and white tea to ward off free radicals. Samples were made into four types of kombucha, consisting of control kombucha (without tea), as well as green tea, black tea, and white tea kombucha. The highest antioxidant activity carried out using the DPPH method was obtained by black tea kombucha with percent inhibition and IC50 values of 88,29 ± 2,20% and 30,84 ± 1,34 μL/ml, respectively. Black tea kombucha also had the highest total phenolics and total flavonoids of 921,28 ± 38,08 mg GAE/L kombucha and 421,82 ± 24,05 mg QE/L kombucha respectively. The correlation results showed total phenolics and total flavonoids are directly proportional to the percent inhibition and inversely proportional to the IC50 value in kombucha. The characteristics of kombucha include pH and density which decrease after fermentation.
       
      Teh kombucha merupakan minuman fermentasi dari cairan teh yang mengandung gula oleh kultur kombucha atau SCOBY (Symbiosis Culture of Bacteria and Yeast). Uji total fenolik, total flavonoid, dan aktivitas antioksidan dilakukan untuk menganalisis dan membandingkan kemampuan antioksidan pada kombucha yang berasal dari teh hijau, teh hitam, dan teh putih dalam menangkal radikal bebas. Sampel dibuat menjadi empat jenis kombucha, terdiri dari kombucha kontrol (tanpa teh), serta teh hijau, teh hitam, dan teh putih kombucha. Aktivitas antioksidan tertinggi yang dilakukan dengan metode DPPH didapatkan oleh teh hitam kombucha dengan persen inhibisi dan nilai IC50 masing-masing sebesar 88,29 ± 2,20% dan 30,84 ± 1,34 μL/ml. Teh hitam kombucha juga memiliki total fenolik dan total flavonoid tertinggi masing-masing sebesar 921,28 ± 38,08 mg GAE/L kombucha dan 421,82 ± 24,05 mg QE/L kombucha. Hasil korelasi menunjukkan total fenolik dan total fenolik berbanding lurus dengan persen inhibisi dan berbanding terbalik dengan nilai IC50 pada kombucha. Adapun karakteristik kombucha berupa pH dan massa jenis yang berkurang setelah fermentasi.
       
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      http://repository.ipb.ac.id/handle/123456789/152982
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      • UT - Biochemistry [1466]

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