Biohydrogen Production by Fermentation of Photosynthetic Bacteria Rhodobium marinum and Sanur Isolate.

Abstract

Bioenergy is renewable energy and prospective to be developed. It is not only can reduce our dependence of fueloil, but also can increase safety of national stock energy. Biohydroen is a type of bioenergy which used as fuel cell, industry necessity, and nuclear. This research have focus on production of hydrogen gas by photofermentation of Rhodobium marinum and bacteria consortium of Sanur isolate using glucose 1% as a substrate. This photofermentation was done with R. marinum and Sanur isolate were centrifuged (6000 rpm, 20 minutes). Then each pellet was resuspented in 75 ml production medium which containing glucose 1% on 100 ml serum bottles and be seted on bioreactor (120 rpm, 30ºC , TL light 60 watts). The result shown that R. marinum ability to used glucose 1% to produce 127.311 ml hydrogen gas of 205.25 gas completly that produced (62%) with production rate is 3.81 mmolH2h-1L-1medium. Bacteria consortium of Sanur isolate also can produce 103.61 ml hydrogen gas of 165.25 ml gas completly that produced (63%) with production rate is 2.8 mmolH2h-1L-1medium.