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      • UT - Faculty of Fisheries and Marine Science
      • UT - Aquaculture
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      Aplikasi Probiotik Staphylococcus lentus L1k terhadap Kinerja Produksi dan Respons Imun Ikan Lele (Clarias sp.) yang Diinfeksi Aeromonas hydrophila

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      Date
      2024
      Author
      Rizki, Irene Ailsa
      Yuhana, Munti
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      Abstract
      Produksi ikan lele yang terus meningkat memerlukan pengembangan budidaya dengan sistem intensif, namun penyebaran penyakit Motile Aeromonas Septicemia yang disebabkan oleh bakteri Aeromonas hydrophila dalam budidaya intensif masih menjadi masalah. Pencegahan penyakitnya dapat dilakukan dengan meningkatkan respons imun ikan melalui pemberian probiotik. Penelitian ini bertujuan menguji pengaruh aplikasi probiotik Staphylococcus lentus L1k dengan dosis yang berbeda terhadap kinerja produksi dan respons imun ikan lele (Clarias sp.) yang diinfeksi Aeromonas hydrophila pada dosis yang efektif. Penelitian terdiri atas lima perlakuan yaitu K+ (tanpa pemberian probiotik dan diuji tantang), K- (tanpa pemberian probiotik dan tanpa uji tantang), A (L1k 104 CFU mL-1 1% dan diuji tantang) B (L1k 106 CFU mL-1 1% dan diuji tantang), dan C (L1k 108 CFU mL-1 1% dan diuji tantang). Ikan uji memiliki bobot awal (29,49 ± 0,04) g dipelihara selama 30 hari dan diberi pakan suplementasi, kemudian diuji tantang dengan bakteri A. hydrophila selama 10 hari. Hasil penelitian menunjukkan pemberian probiotik Staphylococcus lentus L1k mampu meningkatkan kinerja produksi dan respons imun ikan lele (Clarias sp.) yang diinfeksi bakteri Aeromonas hydrophila dengan dosis terbaik pada pakan dengan penambahan bakteri Staphylococcus lentus L1k dengan dosis 104 CFU mL-1.
       
      The continuously increasing production of catfish requires the development of intensive aquaculture systems. However, the spread of Motile Aeromonas Septicemia disease caused by the bacterium Aeromonas hydrophila in intensive aquaculture remains a problem. Disease prevention can be achieved by enhancing the fish's immune response through the administration of probiotics. This study aims to test the effect of the application of Staphylococcus lentus L1k probiotics at different doses on the production performance and immune response of catfish (Clarias sp.) infected with Aeromonas hydrophila at an effective dose. The study consists of five treatments: K+ (no probiotic administration and challenged), K- (no probiotic administration and not challenged), A (L1k 104 CFU mL-1 1% and challenged), B (L1k 106 CFU mL-1 1% and challenged), and C (L1k 108 CFU mL-1 1% and challenged). The test fish had an initial weight of (29.49 ± 0.04) g and were reared for 30 days with supplemental feed, then challenged with A. hydrophila bacteria for 10 days. The results of the study show that the administration of Staphylococcus lentus L1k probiotics can improve the production performance and immune response of catfish (Clarias sp.) infected with Aeromonas hydrophila, with the best dose being 104 CFU mL-1 when added to the feed.
       
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      http://repository.ipb.ac.id/handle/123456789/135561
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      • UT - Aquaculture [2191]

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      Indonesia DSpace Group 
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