Antibacterial Activity of Microencapsulation of Trigona spp. Propolis Pandeglang after Following Exposure to Cattle Rumen Fluid.

Abstract

The use of antibiotics to overcome diarrhea in cattle causes some problems related to microbe resistence and antibiotic residue. The alternative solution is using natural antibiotic like propolis. Aim of this research is study the effect of propolis microencapsulation on release time of propolis following exposure to cattle rumen fluid. Trigona spp. Propolis used on this research collected from Pandeglang, West Java. Propolis was extracted with maseration method. Then, condensed extract propolis was microencapsulated using maltodextrin as filler. Concentration of propolis were 2% and 4%. The mixture was dried using vaccum drying method. Then, propolis microcapsule was put into hard gelatine capsule size 00 and 1. Capsules were then tested with endurance test to cattle rumen. After that, antibacterial activity of propolis was tested using well diffusion method. Rendement of propolis extract produced is 8.81%. Propolis microcapsule has brown-yellow colour. Antibacterial activity of propolis was still detected until 24 hour after cattle rumen fluid treatment. Microencapsulation of propolis has a potency to slow down release of propolis active compounds in cattle rumen. The effective value of the highest inhibition compared to propolis X-brand on 227 mg of capsule size and propolis 4% is 160.69%.