Optimation of N-acetylglucosamine Production from Chitin by Aspergillus rugulosus 501 Fermentation .

Abstract

Chemical hydrolysis of chitin into N-acetyl-glucosamine (GlcNAc) using strong acid such as HCl will damage environment and hard. Enzymatic hydrolysis using chitinase is an alternative method to overcome such problems. Fermentation was done by A. rugulosus 501 (1% v/v) using GlcNAc production medium with variation initial pH (4, 5, 6, and 7) and nitrogen source (bacto peptone, yeast extract, ammonium sulphate, and urea). Samples were to be at day 0; 5; 6; 7; 8; 9 10 and measured using Reissig method. Chemical of GlcNAc tied by calium tetraborat then added reagent p-dimetilaminobenzaldehida (DMAB) and absorbtion measured at 584 nm wave. The obtained GlcNAc were to be concentrated using evaporator and freeze dryer. The results showed that GlcNAc concentration obtained was very low. The optimal production (2,228 μg/mL) were observed at the initial pH 4 and urea as nitrogen source at day 10.