Kombinasi Isolat Bakteri Simbion Rayap Dengan Isolat Bakteri Rumen Dalam Meningkatkan Nilai Guna Pakan Sumber Serat

Abstract

The aim of this experiment was to determine the best combination between termite bacteria and rumen bacteria to degradate fibrous feeds. This experiment used randomized block design with factorial design (3x6) and 3 replications. The first factor was combination between termite bacteria and rumen bacteria {T1 (A + SE 511), T2 (A + SE 512), T3 (A + SE 513), T4 (D + SE 511), T5 (D + SE 512), and T6 (D + SE 513)}, and the second factor was fibrous feeds (napier grass, rice straw and palm press fibre). A (SB 53 5(3)1) and D (SC 51 5(2)) were isolates of termite bacteria and SE 511, SE 512, SE 513 were isolates of rumen bacteria. Variables measured in this experiment were concentrations of NH3 and VFA, and degradabilities of dry matter (DM) and organic matter (OM), digestibilities of DM and OM. Data were analyzed by analysis of variance (ANOVA) and differences among treatments were tested by contrast orthogonal when there was a significant effects of treatment on variables measured. The results showed that differences in fibrous feeds significantly affected all variables measured (P<0.01). Rice straw had higher (P<0.01) VFA concentration than napier grass and palm press fibre. Napier grass and rice straw had higher (P<0.01) NH3 concentration than palm press fibre. Degradability of DM and OM of napier grass were higher than those of rice straw and palm press fibre (P<0.01). Differences in combination between termite bacteria and rumen bacteria significantly affected digestibilities of DM and OM. Treatments of T2, T4, T5 and T6 had higher (P<0.05) digestibility of DM than T1 and T3. Treatments of T4, T5, and T6 had higher digestibility of OM than T1, T2, and T3. It is concluded that the combination between termite bacteria and rumen bacteria have synergic relationship in degradating fibrous feeds. The best combination are T4, T5 and T6 in digesting fibrous feeds with the best isolate of termite bacteria is isolate D {SC 51 5(2)}. In comparison to napier grass, coculture between termite and sheep rumen bacterial isolates for 6 hour incubating period has improved fermentability and digestibility of rice straw, however, no such improvement was obtained in fermentability and digestibility of palm press fibre.