Formulasi Ekstrak Etanol Jamur Kancing (Agaricus bisporus) dan Cangkang Telur Ayam sebagai Hidrogel Antibakteri

Abstract

Diabetic foot ulcer diperparah oleh adanya produksi eksudat berlebih yang mengandung media pertumbuhan bagi bakteri. Penelitian ini bertujuan menentukan formula, menganalisis sifat fisikokimia, dan aktivitas antibakteri hidrogel ekstrak etanol jamur kancing dan cangkang telur ayam. Metode penelitian terdiri atas preparasi cangkang telur ayam dan jamur kancing, ekstraksi jamur kancing, formulasi hidrogel, uji fisikokimia, dan uji antibakteri. Formulasi hidrogel menggunakan konsentrasi ektrak jamur kancing 6,4% serta cangkang telur ayam 5% (F1), 10% (F2), dan 20% (F3). Hidrogel yang dihasilkan memiliki pH 7, fraksi gel 19,44-33,66%, dan swelling index sebesar 16,19-59,94%. Aktivitas antibakteri hidrogel terhadap S. aureus, S. epidermidis, E. coli, dan P. aeruginosa tergolong lemah hingga sedang. Simpulan penelitian ini yaitu F1 (5% cangkang telur) merupakan hidrogel terbaik berdasarkan aktivitas antibakteri terhadap S. aureus, S. epidermidis, dan E. coli.

Diabetic foot ulcers are exacerbated by the overproduction of exudate, which contains a growth medium for bacteria. This research aimed to determine the formula, physicochemical characteristics, and antibacterial activity of hydrogel ethanol extracts of button mushrooms and chicken eggshell. The research steps consisted of preparation of chicken eggshell and button mushrooms, extraction of button mushrooms, hydrogel formulations, physicochemical tests, and antibacterial tests. All of hydrogel formulations used a 6,4% concentration of button mushroom extract and chicken eggshell by 5% (F1), 10% (F2), and 20% (F3). Hydrogel has a pH of 7, gel fraction with a value range of 19,44-33,66%, and swelling index of 16,19-59,94%. Hydrogel has antibacterial activity against S. aureus, S. epidermidis, E. coli, and P. aeruginosa, which is classified as weak to moderate. The conclusion of this research was that F1 consisting of 5% chicken eggshell was the best hydrogel based on antibacterial activity against S. aureus, S. epidermidis, and E. coli.