Fermentabilitas dan Kecernaan Pakan yang Ditambahkan Slow Release Urea-Nitrat In Vitro

Abstract

Penelitian ini bertujuan menguji pengaruh penambahan slow release urea-nitrat dalam ransum secara in vitro. Penelitian ini menggunakan rancangan acak kelompok 5 perlakuan dan 4 ulangan. Perlakuan terdiri dari P0 = ransum kontrol; P1 = P0 + urea; P2 = P0 + urea-nitrat; P3 = P0 + urea-nitrat dilapisi dengan molases; P4 = urea-nitrat dilapisi dengan limbah cair sari kurma. Peubah yang diamati adalah kecernaan bahan kering (KcBK), kecernaan bahan organik (KcBO), konsentrasi amonia (NH3), derajat keasaman (pH), volatile fatty acid (VFA) total, sintesis protein mikroba (SPM), total produksi gas, dan gas metan. Data dianalisis menggunakan analysis of variance (ANOVA) dan perlakuan yang berbeda nyata akan dilakukan uji lanjut dengan uji duncan menggunakan statistical product and service solutions (SPSS) 25. Hasil penelitian menunjukkan bahwa perlakuan tidak berpengaruh nyata (P>0,05) terhadap nilai KcBK, KcBO, VFA total, pH, SPM, total produksi gas, dan gas metan. Perlakuan yang berpengaruh nyata (P<0,05) adalah konsentrasi amonia (NH3). Simpulan penelitian ini adalah penambahan slow release urea-nitrat belum mampu menurunkan konsentasi amonia.

This study aimed to examine the effect of the addition slow release urea-nitrate in rations in vitro. This study used a randomized block design with 5 treatments and 4 replications. The treatment consisted of P0 = control ration; P1 = P0 + Urea; P2 = P0 + urea-nitrate; P3 = P0 + urea-nitrate coated with molasses; P4 = urea-nitrate coated with palm juice liquid waste. The observed variables were dry matter digestibility (KcBK), organic matter digestibility (KcBO), ammonia concentration (NH3), degree of acidity (pH), total volatile fatty acid (VFA), microbial protein synthesis (SPM), total gas production, and methane gas. Data were analyzed using analysis of variance (ANOVA) and significantly different treatments would be further tested with Duncan's test using statistical product and service solutions (SPSS) 25. The results showed that the treatment had no significant effect (P>0.05) on the KcBK, KcBO, total VFA, pH, SPM, total gas production, and methane gas. The treatment that had a significant effect (P<0.05) was the concentration of ammonia (NH3). The conclusion of this study was the addition of slow release urea-nitrate has not been able to reduce the concentration of ammonia.