Pengaruh Penambahan Vitamin E terhadapt Performa Sperma Ikan Lele (Clarias gariepenus) Pascakriopreservasi

Abstract

Kriopreservasi merupakan metode penyimpanan spermatozoa dengan bantuan pengencer dan krioprotektan dalam keadaan beku untuk mempertahankan kualitas spermatozoa. Penelitian ini bertujuan menguji efektivitas penambahan vitamin E pada sperma ikan lele (Clarias gariepenus) terhadap kualitas sperma pascakriopreservasi. Ikan uji yaitu ikan lele yang telah matang gonad dan disuntik hormon ovaprim untuk pematangan akhir. Penelitian ini menggunakan lima perlakuan dengan tiga ulangan yaitu, kontrol (sperma segar), DMSO 10%, DE 1 (vitamin E 2 mg/mL), DE 2 (vitamin E 4 mg/mL), DE 3 (vitamin E 6 mg/mL). Parameter yang diamati meliputi motilitas sperma, viabilitas sperma, derajat pembuahan telur dan derajat penetasan telur. Hasil penelitian menunjukkan nilai motilitas, viabilitas dan derajat pembuahan telur pada perlakuan penambahan vitamin E lebih rendah (P<0,05) dari DMSO 10%. Nilai derajat penetasan telur tidak berbeda nyata antar perlakuan.

Cryopreservation is a method of storing spermatozoa with the help of extenders and cryoprotectants in a frozen state to maintain the quality of spermatozoa. This study aims to test the effectiveness of adding vitamin E to the sperm of catfish (Clarias gariepenus) to see the quality of sperm after cryopreservation. The test fish was catfish that had mature gonads and were injected with the hormone ovaprim for final maturation. This study used five treatments with three replications namely, control, DMSO 10%, DE1, DE2, DE3. Parameters observed included sperm motility, sperm viability, degree of egg fertilization, and egg hatching rate. This study used five treatments with three replications, namely, control (fresh sperm), 10% DMSO, DE 1 (vitamin E 2 mg/mL), DE 2 (vitamin E 4 mg/mL), and DE 3 (vitamin E 6 mg/mL). mL). Parameters observed included sperm motility, sperm viability, degree of egg fertilization, and egg hatching rate. The results showed that the values of motility, viability, and degree of fertilization of eggs in the treatment with the addition of vitamin E were lower (P<0.05) than 10% DMSO. The value of the degree of egg hatching was not significantly different between treatments.