Responses on Performance and Minerals Digestibility by Novel Consensus Bacterial 6-Phytase Variant (PHY-G) Supplementation in Broiler Diet
Date
2022-05Author
Sidiq, Fajrin
Ridla, Muhammad
Hermana, Widya
Ghane, Amir Eslami
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Show full item recordAbstract
Plant feedstuffs normally contain phytic acid which poorly hydrolized by
monogastric especially broiler. Further, 80% of phosphorus (P) in plant feedstuff is
complexed with phytic acid. In that case, high amount of inorganic P sources is
needed to fulfill P requirement. Since it has capability to bind with some nutrients
such as bi- or trivalent minerals and amino acids then phytase is widely utilized in
poultry feed to improve the nutrient digestibility. Today, the use of 500-1000 FTU
phytases in poultry feed is widely applied by feed industry and its capability
releasing phytate-bound phosphorus is very well documented.
Novel consensus bacterial 6-phytase variant (PHY-G) is newest 6-phytase
derived from bacterial phytase gene Buttiauxella sp. expressed in Trichoderma
reesei with enhanced functionality for better phytic acids degradation. The present
research was conducted to evaluate the efficacy of PHY-G in different doses (1000,
1500 and 2000 FTU/kg) on high phytic acids content diets which at least contained
0.30% Phytate-P. A total 3,675 male broilers Indian River/IR (105 pens, 35
birds/pen) were provided mixed grain diets in seven treatments with fifteen
replications, they were divided into two phases of rearing which were starter (1 –
21 d) and finisher (22 – 35 d). Treatments were positive control (PC) using standard
diet following IR’s nutrient requirement, negative control 1 (NC1) with nutrient
reduction at 0.21 percent unit calcium (Ca), 0.21 percent units available phosphorus
(AvP), 0.34 percent unit crude protein (CP) and 66 kcal/kg AME, NC2 with nutrient
reduction at 0.23 percent unit Ca & AvP, 0.45 percent unit CP and 75 kcal/kg AME,
followed by NC3 with nutrient reduction at 0.24 percent unit Ca & AvP, 0.52
percent unit CP and 79 kcal/kg AME. PHY-G supplementation with dose 1000,
1500 and 2000 FTU/kg on top of NC1, NC2 and NC3 respectively.
PHY-G supplementation at any level significantly improved body weight
gain/BWG and corrected FCR/McFCR (P<0.05) on starter (1,083 – 1,093 g/bird)
and overall phase (2,482 – 2,532 g/bird) compared to any NCs (1,063-1,084 g/bird
on starter and 2,387 – 2,398 g/bird on finisher). No significant different were
observed on mortality of all treatments but PHY-G supplementation significantly
improved (P<0.05) broiler index/BI (444 - 463) versus NCs (427 - 430) and able to
maintain it equivalent to PC (455). Toe ash was significantly improved (P<0.05) by
all doses of PHY-G (13.28 – 13.56%) compared to NC (12.3 – 12.7%). Apparent
ileal digestibility (AID) of Ca was not affected by PHY-G but 1000, 1500 and 2000
FTU/kg PHY-G supplementation significantly improvemed on AID of P (P<0.05)
which were 64.97%, 75.60% and 78.29% compared to NCs (42.64%, 48.88% and
46.17% for NC1, NC2 and NC3 respectively). This These data indicated that PHYG
supplementation effectively improved broiler growth performance, bone
mineralization and P digestibility at any level of dose on high content of phytic acid
in the diets.
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