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      Aplikasi Sinbiotik Monospesies dan Multispesies untuk Pencegahan Infeksi Aeromonas hydrophila pada Ikan Lele Sangkuriang (Clarias gariepinus)

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      Date
      2021
      Author
      Salsabila, Sarah
      Yuhana, Munti
      Widanarni
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      Abstract
      Penyakit bakterial yang mengancam peningkatan produksi ikan lele salah satunya disebabkan oleh bakteri Aeromonas hydrophila. Penggunaan sinbiotik yang merupakan kombinasi probiotik dan prebiotik menjadi alternatif untuk pencegahan penyakit. Penelitian ini bertujuan untuk menguji pengaruh probiotik Bacillus cereus BR2 dengan prebiotik madu (sinbiotik monospesies), probiotik Staphylococcus lentus L1k dengan prebiotik madu (sinbiotik monospesies), probiotik B. cereus BR2 dan S. lentus L1k dengan prebiotik madu (sinbiotik multispesies) yang diberikan melalui pakan untuk pencegahan infeksi A. hydrophila pada ikan lele sangkuriang (Clarias gariepinus). Ikan uji dengan bobot awal (17,43 ± 0,22) g dipelihara selama 30 hari dan diberi pakan suplementasi, kemudian diuji tantang dengan bakteri A. hydrophila selama tujuh hari. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu perlakuan A (BR2 1% + madu 0,5% + diuji tantang), B (L1k 1% + madu 0,5% + diuji tantang), C (BR2 0,5% + L1k 0,5% + madu 0,5% + diuji tantang), kontrol positif (tanpa probiotik dan prebiotik + diuji tantang), dan kontrol negatif (tanpa probiotik dan prebiotik + tanpa diuji tantang). Hasil penelitian menunjukkan sinbiotik monospesies dan multispesies mampu meningkatkan tingkat kelangsungan hidup, kinerja pertumbuhan, dan respons imun ikan lele untuk pencegahan infeksi A. hydrophila dengan hasil terbaik terdapat pada perlakuan sinbiotik multispesies.
       
      One of the bacterial diseases that threaten the increase in catfish production is Aeromonas hydrophila. The used of synbiotics which is a combination of probiotics and prebiotics is an alternative for disease prevention. This study aimed to examine the effect of probiotic Bacillus cereus BR2 with prebiotic honey (monospecies synbiotic), probiotic Staphylococcus lentus L1k with prebiotic honey (monospecies synbiotic), probiotic B. cereus BR2 and S. lentus L1k with prebiotic honey (multispecies synbiotic) through feed for the prevention of A. hydrophila bacterial infection to sangkuriang catfish (Clarias gariepinus). The fish size (17,43 ± 0,22) g were reared for 30 days and given supplementary feed, then challenged with A. hydrophila bacteria for seven days. This study consisted of five treatments and three replications i.e. treatment A (BR2 1% + honey 0,5% + challenged), B (L1k 1% + honey 0,5% + challenged), C (BR2 0,5% + L1k 0,5% + honey 0,5% + challenged), positive control (without probiotics and prebiotics + challenged), and negative control (without probiotics and prebiotics + no challenged). The results showed that monospecies and multispecies synbiotic could increase survival rate, growth performance, and immune respons of catfish to prevent of A. hydrophila infection with the best result found in the multispecies synbiotic treatment.
       
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      http://repository.ipb.ac.id/handle/123456789/110102
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      • UT - Aquaculture [2201]

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