Efektivitas Ekstrak Daun Kemangi Untuk Menurunkan Kadar Gas NH3 dan H2S Ekskreta Puyuh (Coturnix coturnix japonica)

Abstract

ABSTRACT

This study examine the effect of basil leaf extract on the reduction of ammonia gas (NH3) and hydrogen sulphide gas (H2S) quail excreta (Coturnix coturnix japonica) and their optimal composition. Basil leaf extraction maceration method by soaking simplisia on ethanol 96% with comparison ratio 1:10. Antibacterial activity test used disc diffusion method to determine the activity of antibacterial agents. The antibacterial agent using non-antibiotic paper disc and basil leaf. The inoculum contains 106 mL-1 bacterial cells S.aureus and E.coli are spreads on the MHA media. The diluted extract with DMSO until 200 mg mL-1, 150 mg mL-1, 100 mg mL-1 and 50 mg mL-1. Petri dish was incubated at 37oC for 24 hour. This study used a completely randomized design trial with one factor of basil leaf extract with five treatments with a level of 200 mg mL-1, 150 mg mL-1, 100 mg mL-1, 50 mg mL-1, and one treatment without section. This study repeated all treatments three times. This study indicate that the additional basil leaf extract with a maximum concentration 200 ppm optimal can reduce NH3 and H2S gas levels compared to controls were not given additional extract.

Keywords: excreta, basil leaf extract, NH3, H2S