Identifikasi Keragaman Gen Miostatin pada Domba Lokal

Abstract

Myostatin is a member of tranforming grown factor B superfamily, know as growth and differentiation factor, which is essensial for proper regulation of skeletal muscle mass in wide range vertebrate species. To pursue potential agricultural application of increasing muscle mass by inhibition of myostatin activities, the aim of this study was to identify the variants of myostatin gene in the Indonesian local sheeps. A total of 151 DNA samples belonging to eight different areas that are Bogor Ciomas (20), Bogor Jonggol (35), Margawati (18), Indramayu (30), Dinggala (24), MAdura (8), Sumbawa (8), and Rote (8) were analyzed with Polymerase Chain Reaction-Single Starnd Conformation Polymorphism. The Myostatin (GDF 8) amplified product was 688 bp(base pair)