Studi In Vitro Biakan Sel Endotel Kelinci pada Media Cair dan Semi Padat

Abstract

Endothelial cells derived from femoralis artery of rabbit were succesfully cultured in vitro in liquid medium of Dulbeco modified essential medium-Ham’s F-12 (DME/F-12) and semi solid medium of collagen gel. The cells cultured in liquid medium grew and formed colony with a pattern of monolayer sheet. They were round to oval and spindle in shape. The cells were grown in collagen gel by using floating and gel-embedded system to show their three-dimensional structure. The growth pattern of the cells cultured in both collagen gel systems showed a formation of irregular fibrous-like structure, elongated cells and colony with duct-like structure as well as radiating projection pattern and formation of a large lumen like structure. Histological findings using Haematoxylin and Eosin staining showed development of a compact cell colony which formed a monolayer sheet with a cell layer of fibroblast and a thin collagen layer. Immunohistochemically, endothelial cells showed positive reaction for anti-Factor VIII Related-Ag (F VIII R-Ag), with smooth fiber pattern in cytoplasm running along the cell border.